
| 30 seconds at 92°C | Denatures template (cell) DNA | x 30 (30 "cycles") |
| 30 seconds at 65°C | Allows primers to bind specifically ("anneal") to complementary regions in template DNA | |
| 60 seconds at 72°C | Allow Taq polymerase to extend the primers, making 2 complementary strands of DNA ("extension") |