The "One Step Growth Curve"

Here are your samples:

To measure the titre (concentration) of bacteriophage particles in your two samples, you need to make serial 10-fold dilutions.
How are you going to do this? (click on the correct option below):

Method 1: Dispense 9.9ml phage dilution buffer into each of 6 dilution tubes. Label the tubes: 10-1, 10-2, etc. to 10-6. Add 0.1ml phage suspension to the 10-1 tube, mix. With a clean pipette, transfer 0.1ml to the 10-2 tube and mix. Repeat this procedure through the whole dilution series.	Method 2: Dispense 0.9ml phage dilution buffer into each of 6 dilution tubes. Label the tubes: 10-1, 10-2, etc. to 10-6. Add 0.1ml phage suspension to the 10-1 tube, mix. With a clean pipette, transfer 0.1ml to the 10-2 tube and mix. Repeat this procedure through the whole dilution series.
Method 3: Dispense 0.9ml phage dilution buffer into each of 6 dilution tubes. Label the tubes: 10-1, 10-2, etc. to 10-6. Add 0.9ml phage suspension to the 10-1 tube, mix. With a clean pipette, transfer 0.1ml to the 10-2 tube and mix. Repeat this procedure through the whole dilution series.	Method 4: Dispense 1ml phage dilution buffer into each of 6 dilution tubes. Label the tubes: 10-1, 10-2, etc. to 10-6. Add 0.1ml phage suspension to the 10-1 tube, mix. With a clean pipette, transfer 0.1ml to the 10-2 tube and mix. Repeat this procedure through the whole dilution series.