- Place the slide on a staining rack.
- STAIN the slide with crystal violet for 1-2min.
- Pour off the stain.
Note: fingers stain Gram-positive - use forceps! - Flood slide with Gram's iodine for 1-2min.
- Pour off the iodine.
- Decolourize by washing the slide briefly with acetone (2-3 seconds).
- Wash slide thoroughly with water to remove the acetone - do not delay with this step.
- Flood slide with safranin COUNTERSTAIN for 2min.
- Wash with water.
- Blot excess water and dry in hand over bunsen flame.
